Determination of methadone, 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine, 2-ethyl-5-methyl-3,3-diphenylpyraline and methadol in meconium by liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry

下载全文     加入收藏夹         被引：

This paper details a validated liquid chromatography atmospheric pressure chemical ionization tandem mass spectrometry (LC-APCI-MS/MS) method for the quantification of methadone, and its metabolites 2-ethylidene-1,5-dimethyl-3,3-diphenylpyrrolidine (EDDP), 2-ethyl-5-methyl-3,3-diphenylpyraline (EMDP) and methadol in human meconium. Limits of detection (LOD) were determined to be 1.0 ng/g for methadone, EDDP and EMDP and 2.5 ng/g for methadol. The limits of quantitation (LOQ) for methadone, EDDP, EMDP were 5 and 25 ng/g for methadol. Linearity ranged from 5.0 to 500 ng/g. Following solid-phase extraction, no matrix effect was observed. This method proved to be suitable for the quantification of methadone, EDDP and EMDP and the semi-quantitation of methadol in meconium. Literature review revealed no other published LC-APCI-MS/MS method for the detection of methadone and its three main metabolites in meconium specimens. Published by Elsevier B.V.

作 者	Choo, RE (NIDA, Chem &) ； Murphy, CM (Drug Metab Sect, Intramural Res Program, NIH, Baltimore, MD 21224 USA) ； Jones, HE Huestis, MA
刊 名	Journal of chromatography. B, Analytical technologies in the biomedical and life sciences   2005 年814卷2期
关键词	methadone meconium LC-APCI-MS/MS STEREOSELECTIVE DETERMINATION QUANTITATIVE-DETERMINATION ENANTIOSELECTIVE ANALYSIS PRIMARY METABOLITE HUMAN PLASMA COCAINE URINE IMMUNOASSAY ENANTIOMERS SALIVA
MeSH主题词	Hydrogen-Ion Concentration
分类号	R362